7. MODULATION OF DNA DAMAGE (II) Conjugating enzymes, glutathione, antioxidant defences
- Phase II reactions: various enzymes complete the biotransformation of toxic compounds started by the phase I enzymes. Typically, they conjugate the reactive oxidised intermediates to a carrier molecule, such as glutathione, GSH.
Defensive mechanisms have evolved that prevent oxidation by quenching or inactivation free radicals or reactive oxygen.
- Glutathione is a very abundant sulphydryl-rich tripeptide. It is a powerful antioxidant, becoming oxidised by reactive oxygen to the dimer, GSSG. It is then regenerated, in a continuous cycle.
(See e-lecture Oxidative stress and vitamin E on glutathione and protective enzymes.)
- Antioxidant enzymes
- Superoxide dismutase (SOD) - converts superoxide to hydrogen peroxide - contains copper and zinc
Catalase (breaks down hydrogen peroxide) - contains iron
- Glutathione S transferase (GST) and GSH peroxidase (supports vitamin E in protection against lipid peroxidation) - contains selenium
- (Thioredoxin reductase maintains antioxidant activity of vitamin C)
Dietary antioxidants may also play a role; see Other micronutrients electure .
Modulation of DNA damage